丝氨酸蛋白酶研究进展

On serine protease

综述了丝氨酸蛋白酶的结构与功能、蛋白质工程、表达系统和应用。丝氨酸蛋白酶是一类以丝氨酸为活性中心的重要的蛋白水解酶,在生物有机体中发挥着重要而又广泛的生理作用,具有广泛的研究和应用价值。它们的活性部位都含Ser、His、Asp,并具有相同的催化机制。丝氨酸蛋白酶超家族成员执行多种多样的生理功能,在很多致病过程以及细胞内的信号转导等方面起着重要作用。正是由于丝氨酸蛋白酶在结构上的微小变化,从而引起了其在功能上的进化。

This paper reviews the serine proteases＇ structures, functions, protein engineering, expression system, and applications. Serine protease is a group of proteolytic enzymes that are important for its potential applications. All serine proteases have serine, histidine and aspartic acid in their active domain, and same catalytic mechanism. The members in the serine protease super family serve a wide range of physiological functions, especially in the pathogenesis of many diseases and intracellular signal transduction. Any minute alternations in their structures can result in significant functional changes.