低分子量肝素抑制骨肉瘤LIVIN基因表达并诱导细胞凋亡的实验研究

The Experiment Study on LMWH Inhibiting the Expression of Livin and Inducing the Apoptosis of Osteosarcoma Cells

目的研究低分子量肝素(LMWH)对骨肉瘤Livin基因表达及细胞凋亡的影响。方法体外培养骨肉瘤细胞系MG-63,应用MTT法检测不同浓度LMWH不同时间段对骨肉瘤细胞系MG-63生长的影响,流式细胞仪检测细胞凋亡率的变化,SABC免疫组化法分析不同浓度LMWH对骨肉瘤细胞Livin基因表达的影响。结果LMWH可抑制骨肉瘤细胞系MG-63生长,且随着LMWH剂量的增加,MG-63细胞凋亡率明显增加。免疫组化显示,骨肉瘤细胞系MG-63中LIVIN的表达较用药前明显下降。结论LMWH对骨肉瘤细胞系MG-63有明显的抑制作用,其作用可能通过抑制抗凋亡基因Livin的表达,促进肿瘤细胞的凋亡而实现。

Objective To investigate the inhibitory effects of LMWH suppressing the expression of Livin and inducing the apoptosis of the osteosarcoma cells. Methods Osteosarcoma cells line MG -63 was cultured in vitro. MTT assay and flow cytometry were used to study the effect of Livin with different concentration suppressed the proliferation and induced apoptosis in osteosarcoma cells line MG - 63. The expression of livin of osteosarcoma cells line MG - 63 was analyzed by the immunohistochemical method. Results Low molecular .weight heparin could inhibit the growth of osteosarcoma cell line MG -63. With the LMWH＇s dose increasing, the apoptosis rate was increased significantly. Immunohistochemical method showed that the expression of livin of osteosarcoma cells line MG -63 declined obviously than that before medication. Conclusioa LMWH has very strong anti- tumor effect in vitro, the possible mechanisms of LMWH anti - tumor effect are associate with the effect of suppressing the expression of livin and inducing cell apoptosis.