TNF-α和IL-8在兔创伤性急性肺损伤中的意义

THE SIGNIFICANCE OF INCREASED TNF α AND IL 8 IN RABBITS WITH TRAUMATIC ACUTE LUNG INJURY

为探讨肿瘤坏死因子α（ＴＮＦ－α）和白细胞介素８（ＩＬ－８）在创伤性急性肺损伤（ＡＬＩ）发生发展中的可能作用，采用兔胸部撞击致创伤性ＡＬＩ模型，利用酶联免疫法观察伤后血浆及支气管肺泡灌洗液（ＢＡＬＦ）中ＴＮＦ－α及ＩＬ－８含量的变化，辅以动脉血氧分压（ＰａＯ２），肺湿／干比值和病理检查。结果显示：伤后兔出现急性呼吸衰竭、严重肺水肿，肺内大量炎性细胞聚集、浸润；血浆、ＢＡＬＦ中ＴＮＦ－α和ＩＬ－８含量均明显升高，且与肺湿／干比值增加和ＰａＯ２下降呈显著相关。提示：ＴＮＦ－α及ＩＬ－８参与了创伤性ＡＬＩ的发生发展。

To investigate the possible roles of tumor necrosis factor a (TNF α) and interleukin 8(IL 8) in the onset and development with traumatic acute lung injury (ALI) of rabbit blunt chest trauma model, the present study was designed to determine the continuous changes of the contents of TNF α and IL 8 in plasma and bronchoalveolar lavage fluid (BALF) after trauma, by the use of ELISA kit, the concomitant changes of arterial oxygne partial pressure, wet/dry weight value of lung and pathologic following changes. The results revealed that rabbits showed acute respiratory failure and serious pulmonary edema after blunt chest trauma while a lot of inflammatory cells aggregated and infiltrated in the lung. And both the contents of TNF α and IL 8 were found to have a marked increase correlation with increased wet/dry weight of lung and decreased PaO 2. The results suggested that TNF α and IL 8 take an important role on the development of traumatic ALI.