摘要
应用RT-PCR技术从人B淋巴瘤细胞系Raji中克隆到B7-1(CD80)cDNA,并经测序证实。将其插入至真核表达载体pLXSN中,用脂质体转染小鼠黑色素瘤细胞B16,G418筛选,并经流式细胞仪检测,得到了细胞表面表达B7-1的重组克隆。PCR从其基因组DNA中扩增到B7-1基因,提示B7-1cDNA已整合至宿主细胞的基因组DNA中。本文为研究B7-1在肿瘤免疫中所起的作用奠定了基础。
B7 1(CD80) cDNA was cloned by RT PCR from human B lymphma Raji cell line and confirmed by DNA sequencing.The recombinant expressing vector was constructed by inserting the B7 1 cDNA into pLXSN vector.After transfecting by lipofectamine and selecting with G418,the B7 1 transfected recombinant clone was obtained.It was confirmed that the B7 1 cDNA had been integrated into the genomic DNA of B16 B7 cells by PCR analysis.Our study enables us to further investigate the role of B7 1 in tumor immunotherapy.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1997年第3期164-167,共4页
Immunological Journal