摘要
应用ＲＴ－ＰＣＲ技术从人Ｂ淋巴瘤细胞系Ｒａｊｉ中克隆到Ｂ７－１（ＣＤ８０）ｃＤＮＡ，并经测序证实。将其插入至真核表达载体ｐＬＸＳＮ中，用脂质体转染小鼠黑色素瘤细胞Ｂ１６，Ｇ４１８筛选，并经流式细胞仪检测，得到了细胞表面表达Ｂ７－１的重组克隆。ＰＣＲ从其基因组ＤＮＡ中扩增到Ｂ７－１基因，提示Ｂ７－１ｃＤＮＡ已整合至宿主细胞的基因组ＤＮＡ中。本文为研究Ｂ７－１在肿瘤免疫中所起的作用奠定了基础。
B7 1(CD80) cDNA was cloned by RT PCR from human B lymphma Raji cell line and confirmed by DNA sequencing.The recombinant expressing vector was constructed by inserting the B7 1 cDNA into pLXSN vector.After transfecting by lipofectamine and selecting with G418,the B7 1 transfected recombinant clone was obtained.It was confirmed that the B7 1 cDNA had been integrated into the genomic DNA of B16 B7 cells by PCR analysis.Our study enables us to further investigate the role of B7 1 in tumor immunotherapy.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1997年第3期164-167,共4页
Immunological Journal
