100例脑梗死患者血小板糖蛋白Ⅰbα基因多态性测定及临床意义的研究

Gene Polymorphism Analysis and Clinical Significance of Platelet Glycoprotein Ⅰbα in 100 Cerbral Infarction Patients

目的探讨血小板糖蛋白Ⅰbα基因HPA2多态性在脑梗死发病中的作用。方法以分子生物学技术采用PCR方法测定100脑梗死患者与正常对组血小板糖蛋白Ⅰbα基因HPA2多态性。结果100例脑梗死患者组、大动脉粥样硬化型脑梗死患者组及小动脉闭塞型脑梗死患者组(TOAST分型)与相应对照组之间GPⅠbαHPA2基因型频率的分布差异无显著性;大动脉粥样硬化型中杂合突变型比例(16.1%)要高于小动脉闭死型中杂合突变型比例(10.1%),但差异无显著意义。结论GPⅠbαHPA2基因杂合突变型可能并非是脑梗死的危险因素。

Objective To study the effects ofgene polymorphism ofplatelet glycoprotein Ibα to the onset of cerbral infarction.Methods 100 cerbral infarction patients and controls were enrolled in the study.Genotyping for glycoprotein Ibα HPA2 polymorphism was performed by polymerase chain reaction （PCR） amplification. Results There were no statistically significant differences of the GP Ibα HPA2 genotype between Large-vessel subtype group,Small-vessel subtype group,both Large and Small-vessel group（TOAST criteria） and corresponding controls.The heterozygote genotype of GP Ibα HPA2 was more frequent in Large-vessel subtype group（16.1%） than that in Small-vessel subtype group（10.1%）,but the difference was not statistically significant.Conclusion The polymorphism of GP Ibα HPA2 genotype might not be genetic risk factor of cerebral infarction.