高效液相色谱-蒸发光散射检测法测定通迪胶囊中人参皂苷Rg_1和三七皂苷R_1的含量

HPLC-ELSD Determination of ginsenoside Rg_1 and notoginsenoside R_1 in Tongdi Jiaonang

目的：建立HPLC-ELSD法，测定通迪胶囊中人参皂苷Rg1和三七皂苷R1的含量。方法：采用Alltech RP—C18（5μm，4．6mm×250mm）色谱柱，柱温为室温；乙腈一水为流动相，梯度洗脱，流速：1．0ml／min；蒸发光散射检测器检测。漂移管温度105℃，气体流速3．20ml／min。结果：人参皂苷Rg1和三七皂苷R1线性范围分别为0．97～9．70μg（r=0．9993）和0．36～3．60μg（r=0．9991），平均回收率分别为99．08％（RSD=0．91％）和97．53％（RSD=1．04％）。结论：本法简便快捷。准确度高，重复性好．可作为本制剂的质控方法。

Objective： To establish a HPLC - ELSD method for determination of ginsenoside Rg1 and notoginsenoside R1 in Tongdi Jiaonang.Methods： Chromatography was performed on a Alltech RP- C18 column（5 μm,4.6mm × 250mm） at room temperature. Acetonitrile- water as the mobile phase in gradient elute; flow rate was 1.0ml/min. ELSD was used as detector with gas flow rate of 3.20 ml/min and drift tube temperature of 105℃ .Results：The linear ranges of ginsenoside Rgl and notoginsenoside R1 were 0.97 -9.70μg（r = 0.9993）and notoginsenoside R1 were 99.08% （RSD = 0.91% ） and 97.53% （RSD = 1.04% ）, respectively. Conclusion：The method is sample, rapid, accurate and reproducible and can be used to enhance the quality control of this drug.