薄层层析-紫外分光光度法测定丹参注射液中原儿茶醛的含量

Content determination of protocatechuic aldehyde in Danshen Injection by TLC-UV method

目的：建立薄层层析-紫外分光光度法测定丹参注射液中原儿茶醛的含量。方法：以原儿茶醛为对照品，二氯甲烷-乙酸乙酯-甲酸（8：5：0.8）为展开剂，分离得到原儿茶醛，采用紫外分光光度法，检测波长281nm，测定原儿茶醛的含量。结果：原儿茶醛在0.50-4.50μg／ml范围内线性关系良好，r=0.9997。平均含量为0.202mg／ml，加样回收率为98.0％～103.0％。结论：实验结果表明，薄层层析-紫外分光光度法测定丹参注射液中原儿茶醛的含量，方法操作简便、准确度高、精密度好，可用于丹参注射液的质量控制。

Objective： To separate and determine the content of protocatechuic aldehyde in Danshen Injection by TLC-UV method. Methods： Using protocatechuic aldehyde as reference substance, the content of protocatechuie aldehyde in Dan- shen Injection was determined by TLC-UV with dichloromethane-acetic ether-acetic ether（8：5：0.8） as the developing solvent and the detective wavelength of 281 nm.Results： Protocatechuic aldehyde had a good linearity in the range of 0.50- 4.50 μg/ml （r=0.999 7）. The average content was 0.202 mg/ml.The recoveries were 98%-103%. Conclusion： The TLC-UV method is simple, rapid and reproducible, and can be used for the quality control of Danshen Injection.