压力超负荷大鼠肾组织脂质过氧化的变化及替米沙坦干预作用

Change of lipid peroxidation of nephric tissue in pressure-overloaded rat model as well as intervention of telmisartan

目的:探讨腹主动脉缩窄法模拟压力超负荷大鼠模型肾组织脂质过氧化、肾功能改变及替米沙坦干预作用.方法:将36只Wistar大鼠随机等分成3组:假手术组(SG),腹主动脉缩窄组(OG),替米沙坦+腹主动脉缩窄组(TM+OG),每组12只.采用腹主动脉缩窄法制备压力超负荷大鼠模型,分别测定大鼠血清肌酐(Cr)和尿素氮(BUN)含量及大鼠肾组织匀浆丙二醛(MDA)、谷胱甘肽(GSH)含量.结果:腹主动脉缩窄组(OG)及替米沙坦+腹主动脉缩窄组(TM+OG)血清Cr,BUN含量及肾组织匀浆MDA水平均较假手术组(SG)增高,但TM+OG低于OG;肾组织匀浆GSH水平OG及TM+OG低于SG,但TM+OG高于OG.结论:腹主动脉缩窄法制备压力超负荷大鼠肾脏脂质过氧化物改变明显且替米沙坦有保护作用.

AIM： To illustrate the change in lipid peroxidation of pressure-overloaded rat model produced by abdominal aorta constriction as well as the intervention effects of telmisartan. METHODS： Thirty-six Wistar rats were divided into 3 groups： sham-operation group （SG）, abdominal aorta constriction group （operation group, OG）, and telmisartan ＋ operation group （TM ＋ OG）, with 12 in each group. Pressure-overloaded rat models were established by using abdominal aorta constriction method. The levels of creatinine （Cr） and urea nitrogen （BUN） in the rats＇ serum as well as those of malonaldehyde （MDA） and glutathion （GSH） of the rats＇ nephric tissue were measured respectively. RESULTS： Compared with SG, the levels of Cr and BUN and that of MDA in the nephric tissue were significantly higher than those in OG and TM ＋ OG. The levels of these indices in TM ＋ OG were lower than that in OG. The level of GSH in OG and TM ＋ OG was lower than that in SG, but higher in TM ＋ OG than in OG. CONCLUSION： The change of the lipid peroxidation in the pressure-overloaded rat model is obvious and telmisartan exerts the protective effects against the changes.