环磷酰胺抗血管生成化疗抑制鼠S180皮下肉瘤血管形成的作用

Effect of cyclophosphamide antiangiogenesis chemotherapy on murine subcutaneous sarcoma S180

目的:研究环磷酰胺(CTX)抗血管生成化疗(ACT)对昆明鼠S180皮下肉瘤血管形成的抑制作用及机理.方法:将荷S180肉瘤(100mm3)鼠随机分成A组(CTX抗血管生成化疗组),B组(CTX标准化疗组)和C组(生理盐水对照组).按实验方案处理后,检测抑瘤率和肿瘤组织微血管密度,透射电镜观察内皮细胞形态;将接种S180细胞的小鼠随机分成A′组(CTX抗血管生成化疗组),B′组(CTX标准化疗组)和C′组(生理盐水对照组)按实验方案给药,计算成瘤率及肿瘤内的微血管密度.结果:A,B组抑瘤率分别为89.3%和89.9%,A,B,C组微血管密度计数分别为(7.6±5.2),(16.5±5.5)和(17.9±4.7)个/mm2,A′,B′,C′组成瘤率分别为43.8%,6.3%和87.5%.结论:CTX抗血管生成化疗抑制鼠皮下肉瘤微血管形成,并抑制肿瘤的形成和生长.

AIM： To observe the effect of cyclophosphamide （CTX） antiangiogenesis chemotherapy （ACT） on the forming of microvessel in murine subcutaneous sarcoma caused by S180 cells. METHODS： Animals with the sarcoma S180 were randomly devided into 3 groups： A, ACT group; B, standard CTX chemotherapy group; C, saline control group. After administration, the microvessel densities of the sarcoma was measured by immunohistochemical method, and the morphological changes of vascellum endothelial cell after different chemotherapy treatments were observed by the transmission electron microscope. And animals implanted with S180 sarcoma cells were also randomly divided into 3 groups： A′, ACT group; B′, standard CTX chemotherapy group; C′, saline control group. After administration, the ratio of tumor formation and the microvessel densities of the sarcoma were calculated. RESULTS： The mean tumor inhibition rates were 89.3% and 89.9% in group A and B, respectively. Immunohistochemical results showed that the microvessel densities in group A, B and C were 7.6 ± 5.2, 16.5 ±5.5 and 17.9 ±4.7/ mm^2 , respectively. And the mean tumor formation rates in group A′, B′and C′were 43.8%, 6.3% and 87.5%, respectively. CONCLUSION： CTX antiangiogenesis chemotherapy can inhibit the formation and growth of the murine subcutaneous sarcoma by inhibiting the microvessel formation.