温莪术油对脂多糖活化巨噬细胞释放一氧化氮的抑制作用

Inhibitory Effect of Volatile Oil from Curcuma wenyujin on NO Production in LPS-Activated Mouse Macrophage

研究温莪术挥发油及其主要有效成分对活化巨噬细胞释放一氧化氮的抑制作用.方法:以脂多糖(LPS)诱导小鼠单核巨噬细胞RAW 264.7释放NO,通过Griess法测定培养上清中NO的释放量并计算抑制率,通过MTT法评价细胞毒性.结果:温莪术挥发油在1-10μg.mL-1浓度范围内对活化的小鼠巨噬细胞释放NO具有明显的抑制作用,并呈现良好的剂量依赖关系.其主要有效成分呋喃二烯、莪术烯、吉马酮与莪术醇对脂多糖诱导的小鼠巨噬细胞释放NO表现出显著的抑制活性,IC50分别为6.5、5.8、9.4、9.9μmol.L-1.结论:呋喃二烯、吉马酮、莪术烯、莪术醇对活化的小鼠巨噬细胞释放NO具有明显的抑制作用,以上结果可为温莪术油的临床应用提供一定的理论基础和科学依据.

Objective：To study the inhibitory effect of volatile oil from Curcuma wenyujin and its main components on the release of nitro oxide in activated mouse macrophage. Methods ： NO production is induced by LPS and determined by Griess assay. Cytotoxicity is evaluated by MTT assay. Results： Volatile oil from Curcuma wenyujin strongly inhibits the release of nitro oxide at the concentration of 1 -10 μg·mL^-1 and shows good dose-dependency. Its main components exhibit significant inhibitory activity on the NO production. The IC50 values of furanodiene, germacrone, curzerene, curcumenol and curdione are 6.5,5.8,9.4,9.9 and 52μmol·L^-1, respectively. Conclusion： Furanodiene, germacrone, curzerene, curcumenol and curdione can inhibit the NO release in activated mouse macrophage, which may provide some theoretical foundations and scientific basis for the clinical application of Curcuma wenyufin.