乙型肝炎病毒基因型与病毒复制的关系

Relationship between viral genotype and viral replication in hepatitis B virus

目的检测乙型肝炎病毒(HBV)感染者血清HBV基因型和病毒载量(HBV-DNA定量),探讨它们之间的关系。方法采用聚合酶链反应(PCR)-微板核酸杂交-酶联免疫吸附试验(ELISA)检测HBV6种基因型,ELISA法检测血清HBV标记物,荧光定量-聚合酶链反应(FQ-PCR)检测血清HBV-DNA定量值,并对结果进行分析。结果159例标本中能检出基因型者115例,其中B型28例(24.3%),C型56例(48.7%),D型9例(7.8%),混合型22例(19.1%),没有发现A、E和F型。不同基因型的HBV-DNA定量对数值差异无统计学意义(P=0.371>0.05)。不同基因型的HBeAg阳性率差异也无统计学意义(P=0.473>0.05)。结论东莞部分地区HBV基因型以C型为主要检出型,混合型检出率偏高。HBV病毒复制与病毒基因型无明显关系。

Objective To identify the relationship between viral genotype and replication of hepatitis B virus （HBV） in the patients with chronic infected HBV. Methods The viral genotypes were determined by micro-board nucleic acid hybridization-ELISA,and the HBV-DNA levels were detected by quantitative PCR based on fluorescence assay, and the marks of HBeAg were detected by ELISA. Results Genotype B was detected in 28cases（24.3%）, genotype C was detected in 56 cases（48.7%）, genotype D was detected in 9 cases（7.8 % ）, mixed genotypes were detected in 22 cases（19.1%）. None of them had genotyped A or E or F. The HBV-DNA levels in every genotype were not found the statistical difference. The HBeAg positive rates in every genotype were not found the statistical difference. Conclusion The predominant genotype of HBV in the patients with chronic infected HBV in Dongguan is C, and the genotype has nothing to do with the replication of HBV.