人外周血T淋巴细胞胀亡现象的观察

Oncosis in human peripheral blood T lymphocytes

目的:观察人外周血T淋巴细胞的胀亡现象,探讨建立T细胞胀亡检测方法。方法:密度梯度离心法及尼龙棉柱法分离健康成年人外周血T淋巴细胞,分空白组及地塞米松组,培养后观察细胞光镜、荧光镜及电镜形态学,并用流式细胞仪检测胀亡细胞比例变化。结果:①人外周血T淋巴细胞经96小时体外培养,可自然出现典型细胞胀亡形态学改变。②经72小时培养,不同浓度地塞米松组(1×10-6、1×10-5、1×10-4、1×10-3mol/L)T细胞的胀亡率分别为(3.49±0.42)%、(5.17±0.48)%、(8.44±0.72)%、17.93±1.50)%。③在1×10-5mol/L地塞米松作用下,不同培养时间(48、72、96、120小时)T淋巴细胞的胀亡率分别为(0.53±0.10)%、(6.36±0.80)%、(20.60±1.59)%、25.56±1.76)%。结论:人外周血T淋巴细胞存在胀亡现象,地塞米松可诱导人外周血T淋巴细胞胀亡。

Objective：To observe oncosis in human peripheral blood T lymphocytes. Methods：Peripheral blood T lymphocytes of healthy adult was separated with Percoll （ 1. 073 g/ml） and harvested by using nylon column. The cultured cells were divided into control and dexamethasone（DXM） group, and cell morphology was observed through light microscope, electron microscope and fluorescent mi- croscope. And incidence rate of oncosis was analyzed by flow cytometry. Results：（1）Oncosis could be observed in cultured T lymphocyte after 96h. （2）In different concentrations of DXM group （1 × 10^-6, 1×10^-5, 1×10^-4, 1×10^-3mol/L）, The incidence of oncosis T lymphocytes was（3.49±0.42）%, （5.17±0.48）%, （8.44±0.72）%, （17.93 ±1.50）%. （3）During different cultured period（48, 72, 96, 120h）, The oncositic rate of T lymphocytes in DXM group was（0. 53 ±0. 10） %, （6. 36 ±0. 80） %, （20. 60 ± 1.59） %, （25. 56 ± 1.76） %. Conclusion：Oncosis can be seen in human peripheral blood T lymphocytes, and DXM could induce oncosis.