抗鸭胸腺细胞单克隆抗体的制备及其免疫组织化学研究

Prepartion and immunohistochemical study of monoclonal antibodies to duck thymocyte

目的:研究鸭胸腺细胞表面标志。方法:利用杂交瘤细胞融合技术,制备抗鸭胸腺细胞单克隆抗体,并利用免疫组织化学方法研究其反应阳性细胞的组织分布和发生。结果:单克隆抗体C12反应阳性细胞在胸腺,法氏囊和脾脏组织中均有分布,阳性细胞类型不同。结论:C12株杂交瘤细胞分泌的抗体所反应的阳性细胞是成熟的T淋巴细胞和树突状细胞,配体是两者的共同表面标志。

Objective：To investigate the duck thymocyte surface antigens. Methods：Repeated immunization of normal BALB/c mice with separated thymocyte from mature duck result in the production of mAbs which react with duck thymocyte surface antigens. Applying the immunohistochemistry assay, tracing the originating and distributing of lymphocytes which reacted with mAb in spleen, thymus, and bursa of duck different development stages. Results： Five monoclonal antibody-producing hybridomas （ B6, B8, C12, E6, H5 ） were isolated. According to the result of immunohistochemistry assay, there were distributing strained lymphocytes in thymus, bursa and spleen of duck. The stained cells belonged to different populations. Conclusion：The mAb C12 reacted with the common cell surface marker of between mature T lymphocytes and dendritic cells.