摘要
目的:探讨AIRE对外周抗原提呈细胞(APC)TLRs表达的影响。方法:①脂质体法将pEGFPC3-AIRE质粒转染RAW264.7细胞。②共聚焦荧光显微镜观察转染效果。③RT-PCR法检测转染后不同时间点(36、48、72、96小时)RAW264.7细胞上AIRE及TLR1-9的表达水平。结果:①成功将质粒转入RAW264.7细胞,转染效率约为60%～70%。②建立了AIRE转染的RAW264.7细胞,确定转染后72小时为转染最佳时间点。③AIRE转染后72小时,RAW264.7细胞上TLR1、4、5、9表达升高,TLR3、7、8表达降低,转染后96小时,TLR2、6表达升高。结论:AIRE可能通过调控APC上TLRs的表达,调节机体的免疫应答。通过对不同的TLRs的影响,来维持对病原微生物有效应答的同时,维持对自身组织的耐受状态。
Objective ：To explore the effect of autoimmune regulator to TLRs expressions on peripheral antigen presenting cells （APC）. Methods：（1）pEGFPC3-AIRE plasmid was transfected with liposome. （2）Confocal microscopy was used to observe the effect of transfection. （3）RT-PCR assay was used to detect the expressions of AIRE and TLR1-9 in RAW264.7cells at 36, 48, 72, 96 h after transfection. Results：（1）The plasmid was transfected into RAW264.7 cells successfully, and the efficiency of transfection was 60 - 70%. （2）AIRE transfected RAW264. 7 cells were achieved, and the best time was 72 h. （3）At 72 h after transfection, the expressions of TLR1,4,5,9 increased, and TLR3,7,8 reduced. The expression of TLR2,6 increased at 96 h. Conclusion ：AIRE may regulate the immune response by control TLR expression in APC. It maintain the effective response to pathogen and tolerance state to self tissues through the effects to different TLRs.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2008年第1期83-85,89,共4页
Chinese Journal of Immunology
基金
国家自然科学基金资助项目(No.30571724)
