MCP-1在rd小鼠视网膜变性过程中的表达

Expression of MCP-1 in retina of rd mouse

目的探讨单核细胞趋化因子(MCP-1)在rd小鼠视网膜变性过程中的表达。方法RT-PCR反应测定rd小鼠出生后8、10、12、14、16、18d视网膜MCP-1mRNA的表达。原位杂交及免疫组织化学检测高峰期MCP-1mRNA及蛋白在视网膜的定位表达。结果与正常对照鼠相比,MCP-1mRNA在rd小鼠视网膜的表达水平于出生后第8d开始升高,12d达高峰。MCP-1mRNA及蛋白的表达出现于视网膜内层,尤其在内核层细胞及节细胞核周围。结论趋化因子MCP-1在rd小鼠视网膜变性过程中表达升高,提示MCP-1可能在rd小鼠感光细胞凋亡中发挥作用。

Objective Previous studies suggested that chemokines might induce neuron cell death in the central nervous system（CNS） through different signal pathways. The purpose of this study was to investigate the expression of monocyte chemoattractant protein-1 （ MCP-1 ） in the retinal degenerative process of rd mice. Methods Fifty C57BL/6N mice and 50 rd mice were raised in 12-hour light（ 56 -67 lux） and 12-hour black environment alternately, and 50 C57BL/6N mice were raised at normal environment as controls. Expression of MCP-1 mRNA in the retina of rd mouse at postnatal day （P） 8,P10,P12,Pld,P16 and P18 was detected by reverse transcription-polymersase chain reaction （ I/T-PCR ） assay. Location of MCP-1 mRNA and protein at peak time of expression was examined hy in situ hybridization or immunohistochenlical studies. Results Expression of MCP-1 mRNA and protein was noted in peri-nuclei regions in retinal inner nuclear layer and ganglion cell layer, exhibiting a brown or brown-yellow staining. The level of mRNA expression of MCP-1 was increased in the retinas of the rd mice at P8 and P12 compared with normal controls（ P 〈 0.05 ）. Conclusion Expression of chemokine MCP-1 is elevated in retinal degeneration of rd mice,suggesting that MCP-1 may play a role in the photoreceptor degeneration of this animal model.