急性缺血性脑卒中患者外周血Foxp3表达与TGF-β_1的变化

Expression of Foxp3 and Changes of Serum Transforming Growth Factor-β_1 in Patients with Acute Ischemic Stroke

【目的】动态观察急性缺血性脑卒中患者不同时期外周血单个核细胞(PBMCs)中Foxp3基因表达及其与TGF-β1水平的关系,探讨调节性T细胞在急性缺血性脑卒中病理生理演变过程中的作用。【方法】收集正常人和急性缺血性脑卒中患者d1、d3、d7、d14的PBMCs及血清,检测PBMCs中Foxp3 mRNA的表达;同时用ELISA检测血清TGF-β1的水平及变化。分析两者之间的关系。【结果】患者外周血Foxp3 mRNA的表达及外周血清TGF-β1浓度在起病后d1、d3时低于正常对照组(P<0.01);d7逐渐恢复至正常,与正常对照组无明显差异(P>0.05),而到d14则明显高于正常对照组(P<0.01);各组Foxp3 mRNA的表达与血清TGF-β1浓度成正相关(P<0.01)。【结论】调节性T细胞和TGF-β在急性缺血性脑卒中患者病理生理演变过程中起重要作用,急性期参与了缺血后脑组织损害过程而急性后期则可能参与了组织修复的过程。

[Objective]To investigate the correlation of the expression of Foxp3 in peripheral blood mononuclear cells （PBMNCs） and serial changes of serum transforming growth factor-β1 （ TGF-β1 ） in diverse period so as to elucidate theirs roles in the pathogenesis of development of acute ischemic stroke. [Methods] The expression of FOXP3 mRNA in PBMNCs was detected by RT-PCR and serum TGF-β1 was measured by enzymelinked immunosorbent assay（ELISA） at day 1 , 3 , 7 and 14 after the onset of cerebral infarction in 40 patients with acute ischemic stroke and also in 30 healthy controls, furthermore the relationships among them were analyzed. [Results] The level of Foxp3 mRNA and serum TGF-β1 in patients with acute ischemic stroke were significantly decreased at day 1 ,3 （ P〈0.01）, to the nadir at day 3 , and then tended to return toward the control value at day 7 （ P 〉0.05）, increased at day 14 （P〈0.01） . There was a positive correlation between serum level of TGF-β1 and expression of Foxp3 mRNA （P 〈 0. 01） in acute ischemic stroke group. [Conclusion]Treg cell and TGF-β1 may play an important role in the pathophysiology of acute ischemic stroke , playing a damage role at acute stage and a protective role at convalescence stage.