摘要
以凤仙的带芽茎段、茎尖、幼叶为外植体,在附加不同种类及浓度的植物激素的MS培养基上进行快速繁殖,诱导芽的最适培养基为MS+0.5mg·L^-1BA+0.01mg·L^-1IAA+0.01mg·L^-1GA3,芽的增殖培养基为MS+1.0mg·L^-1BA+0.01mg·L^-1IAA+0.01mg·L^-1GA3;增殖系数为6~8倍.根诱导最佳培养基为MS+0.1~0.5mg·L^-1BA+0.005mg·L^-1IAA.
The clonal propagation of Impatiens balsamine L. is developed through culture of stem segment with lateral bud, shoot-tip and young leaf . The optimum medium of shoots induction is MS+0. 5 mg·L^-1 BA+0.01 mg·L^-1 NAA+0.01 mg·L^-1 GA3. Proliferation medium of shoots is MS+1. 0 mg·L^-1 BA +0.01 mg·L^-1 NAA+0.01 mg·L^-1 GA3, proliferation coefficient of shoots can reach 6 to 8. Induction of roots is best in medium of MS+0.1-0.5 mg·L^-1 BA+0.005 mg·L^-1 IAA.
出处
《西北师范大学学报(自然科学版)》
CAS
2008年第1期80-82,87,共4页
Journal of Northwest Normal University(Natural Science)
基金
甘肃省教育厅科研资助项目(0601-27)
关键词
凤仙花
组织培养
快速繁殖
无性繁殖系
Impatiens balsamine L.
tissue culture
rapid propagation
clonal propagation system