凤仙花的组织培养与离体快繁

Tissue culture and rapid propagation of Impatiens balsamine L.

以凤仙的带芽茎段、茎尖、幼叶为外植体，在附加不同种类及浓度的植物激素的MS培养基上进行快速繁殖，诱导芽的最适培养基为MS＋0．5mg·L^-1BA＋0．01mg·L^-1IAA＋0．01mg·L^-1GA3，芽的增殖培养基为MS＋1．0mg·L^-1BA＋0．01mg·L^-1IAA＋0．01mg·L^-1GA3；增殖系数为6～8倍．根诱导最佳培养基为MS＋0．1～0．5mg·L^-1BA＋0．005mg·L^-1IAA．

The clonal propagation of Impatiens balsamine L. is developed through culture of stem segment with lateral bud, shoot-tip and young leaf . The optimum medium of shoots induction is MS＋0. 5 mg·L^-1 BA＋0.01 mg·L^-1 NAA＋0.01 mg·L^-1 GA3. Proliferation medium of shoots is MS＋1. 0 mg·L^-1 BA ＋0.01 mg·L^-1 NAA＋0.01 mg·L^-1 GA3, proliferation coefficient of shoots can reach 6 to 8. Induction of roots is best in medium of MS＋0.1-0.5 mg·L^-1 BA＋0.005 mg·L^-1 IAA.