多器官功能障碍综合征患者外周血单个核细胞SOCS-1／3表达及与预后的关系研究

Study on the relationship between the expression of SOCS-1/3 in the peripheral blood mononuclear cells in patients with multiple organ dysfunction syndrome and their prognosis

目的了解外周血单个核细胞（PBMCs）中细胞因子信号转导抑制因子-1／3（SOCS-1／3）表达与多器官功能障碍综合征（MODS）预后的关系。方法选择32例MODS患者和24例健康志愿者。采用淋巴细胞分离密度梯度离心法分离PBMCs，分别以逆转录-聚合酶链反应及蛋白质免疫印迹法检测PBMCs中SOCS-1／3的mRNA及蛋白表达，分析其与预后及MODS评分的关系。结果MODS组SOCS-1 mRNA表达与正常组比较差异无统计学意义（0．526±0．044比0．466±0．047，P〉0．05），而SOCS-1蛋白表达显著高于与正常组（0．814±0．045比0．479±0．021，P〈0．05）；MODS组中死亡者SOCS-1 mRNA表达显著低于存活者（0．487±0．032比0．532±0．028，P〈0．05），蛋白表达显著低于存活者（0．787±0．029比0．838±0．040，P〈0．05）；MODS患者SOCS-1的mRNA及蛋白表达与MODS评分均呈显著负相关（r1=-0．731，r2=-0．526，P均〈0．01）。MODS组SOCS-3 mRNA表达显著高于正常组（0．993±0．415比0．461±0．046，P〈0．05），SOCS-3蛋白表达显著高于正常组（0．458±0．033比0．403±0．024，P〈0．05）；MODS组中死亡者SOCS-3 mRNA表达显著高于存活者（1．245±0．408比0．805±0．326，P〈0．05），蛋白表达显著高于存活者（0．486±0．021比0．425±0．016，P〈0．05）；MODS患者SOCS-3 mRNA表达与MODS评分呈正相关，但不显著（r=0．468，P〉0．05），SOCS-3蛋白表达与MODS评分呈显著正相关（r=0．783，P〈0．01）。结论MODS中SOCS-1表达可能起到保护组织避免损伤的作用，其表达减少可能提示患者预后不良；SOCS-3的表达可能起损伤作用或增加组织对损伤的易感性，其表达增加可能提示病情严重及预后不良。

Objective To observe the mRNA and protein expression of suppressor of cytokines signaling protein 1/3 （SOCS-1/3） in the peripheral blood mononuclear cells （PBMCs） in patients with multiple organ dysfunction syndrome （MODS）, and to explore the relationship between the SOCS expression and the patients＇ prognosis. Methods Peripheral blood specimens of 32 patients with MODS and 24 healthy volunteers （controls） were collected and the PBMCs were isolated by centrifugation and Ficoll-Hypaque sedimentation. The mRNA of SOCS-1/3 was determined by reverse transcription-polymerase chain reaction （RT-PCR）. And the protein content of SOCS-1/3 was determined by Western blotting. The relationship between the SOCS expression and the patients＇ prognosis was analyzed. Results There was no significant difference in the SOCS-1 mRNA expression between control group and MODS group （0. 526 ± 0. 044 vs. 0. 466±0. 047, P〉0.05）. The SOCS-1 expression of MODS group was significantly higher than that of control group （0. 814 ± 0. 045 vs. 0. 479± 0. 021, P〈0.05）. In the MODS group, the SOCS-1 mRNA expression and protein content of dead patients were significantly lower than those of survived patients （mRNA 0.487±0.032vs. 0.532±0.028, protein 0. 787±0. 029 vs. 0.838±0.040, both P〈0. 05）. There was significant negative correlation between the SOCS-1 mRNA expression and the MODS score （r1 =- 0. 731, P〈0. 01）. There was also significant negative correlation between the SOCS-1 content and the MODS score （r2=-0. 526, P〈0.01）. The SOCS-3 mRNA expression of MODS group was higher than that of control group （0. 993±0. 415 vs. 0. 461±0. 046, P〈0.05）. The SOCS-3 content in the PBMCs of MODS groups was significant higher than that of control group （0. 458±0. 033 vs. 0. 403±0. 024, P〈0.05）. In the MODS group, the SOCS-3 mRNA expression and protein content of dead patients were significant higher than those of survived patients （mRNA 1.245±0.408vs. 0.805±0.326, protein 0.486±0.021 vs. 0.425±0. 016, both P〈0. 05）. There was positive correlation between the SOCS-3 mRNA expression and the MODS score but the correlation was not significant （r= 0. 468, P〉0.05）. And there was significant positive correlation between the SOCS-3 content and the MODS score （r= 0. 783, P〈0.01）. Conclusion SOCS-1 may protect the tissue from further damage while SOCS-3 may cause tissue damage indirectly. The detection of SOCS-1/3 may help to predict the prognosis of MODS.