脑梗死肝阳化风证患者外周血淋巴细胞凝胶图谱分析

Analysis of 2-Dimensional Electrophoregrams of Peripheral Blood Lymphocyte on Patients With Liver Yang Forming Wind Syndrome of Cerebral Infarction

目的建立脑梗死肝阳化风证高分辨率的二维凝胶电泳图谱,从蛋白质组学角度探讨脑梗死中医肝阳化风证的本质内涵。方法设脑梗死肝阳化风证组、健康人对照组、脑梗死阴虚生风证对照组3组,取外周静脉血分离淋巴细胞提取蛋白质,经双向凝胶电泳,考马斯亮蓝染色获取凝胶图谱,PDQuestV7.3.1软件比较分析3组图谱并识别差异表达蛋白质点。结果建立了脑梗死肝阳化风证、健康人组、脑梗死阴虚生风证患者外周静脉血淋巴细胞2-DE图谱,分别获得蛋白质点459个,552个,644个;通过分析比较,发现了脑梗死肝阳化风证与阴虚生风证的相同蛋白质点8个,差异蛋白质点23个。结论提示相类证有共同的物质基础,同病异证有不同的本质内涵,为进一步研究中医肝阳化风证本质内涵奠定了科学基础。

Objective To establish high resolution 2 - dimensional electrophoregrams of Gan Yang Hua Feng syndrome （GYHF） of cerebral infarction, and to use proteomics for investigating essence of GYHF syndrome, Methods To design 3 groups for investigation, the group of GYHF syndrome with cerebral infarction, the group of Yin Xu Sheng Feng syndrome （YX- SF） with cerebral infarction and the normal subjects served as controls. The total proteins from lymphocyte of peripheral ve- nous blood in these 3 experimental groups were extracted, and 2 - dimensional electrophoresis was performed to separate to- tal proteins and stained conventionally by Coomassie brilliant blue G - 250. The differential expressed proteins of these 3 groups were analyzed by PDquest V7.3.1 software. Results High resolution 2 - dimensional electrophoregrams of GYHF syndrome, YXSF syndrome and control group were obtained, and the protein spots were 4.59, 644, and .552 respectively. Twentythree differential and 8 identical protein spots were revealed out from the groups of GYHF and YXSF. Conclusions The results suggest that similar syndromes may have common matter foundation and different syndromes of same disease may have different essence, which will be helpful in further study of GYHF syndrome.