二仙益骨汤治疗去势大鼠骨质疏松症的实验研究

The Experimental Study of ERXIANYIGUTANG on the Prevention of Osteoporosis in Ovariectomized Rats

目的:研究二仙益骨汤治疗去势大鼠骨质疏松症的疗效,并探讨其作用机理。方法:取12周龄的雌性wistar大鼠40只,其中30只打开腹腔去除双侧卵巢,另10只予以单纯打开腹腔不切除卵巢。分组饲养给药12周,测定骨密度、血清中骨钙素(BGP)、降钙素(CT)水平、及骨生物力学指标。结果:经过12周的二仙益骨汤水煎液灌胃治疗,明显提高了去势大鼠的骨密度、血清激素水平,并能改善治疗组大鼠的骨力学性能。结论:二仙益骨汤对去势大鼠的骨质疏松症具有治疗作用,可提高骨密度值,改善骨的生物力学性能。推测其作用可能是通过改变体内激素水平来实现的。

Objective：The experimental study was carried out to prove the efficacy of ERXIANYIGUTANG in ovariectomized rats and to investigate its mechanism. Methods.. After 12 weeks of operation, female Wistar 40 rats were divided into 4 groups, administered saline ,ERXIANYIGUTANG recipe anda D3. Dual energy X-ray absorptiometry （DEXA） was used to measure the bone mineral density （BMD） and the bone miniral contents （BMC） . Serum was collected for the analysis of Ca and phosphorous as well as the level of BGP and CT. Bone biomechanical properties test was performed using a material testing machine. Results： The BMD and BMC of Group B, Group C and Group D were significantly increased corn pared with those in Group A（P〈0.01）. Results showed that the difference between Group B and Group A was significant （P〈0.05） for CT. The load that the femur withstood in three point bending test was also increased in Group B. Compared with Group A （P〈0.01）, the other biomechanieal parameters also inclined. Conclasions：ERXIANYIGUTANG effectively prevent bone loss and obviously increased bone biomeehanical propertie in OVX rats. High level secretion of CT maybe play important roles in ERXIANYIGUTANG treatment of osteoporosis.