荧光光谱法研究β-环糊精与灿烂甲酚蓝的包合作用

Study on the complexation reaction between brilliant cresy blue and β-clodextrin by fluorescence spectrometry

利用灿烂甲酚蓝的荧光强度随β-环糊精浓度的增加呈现规律性的增加,而最大吸收波长及发射波长不变的性质,用Hildebrand-Benesi法测定了β-环糊精与灿烂甲酚蓝包合物的生成常数,确定包合比,并预测了包合物的可能结构。

The inclusion constants was investgated by means of Hildebrand - Benesi double - reciprocal plot which based on the fact that the excitation and emission peak position of Brilliant cresy blue kept unchanged but the emission strength increased disciplinarily with the increasing of β - cyclodextrin concentration. The results showed that the inclusion consisted of oneβ-cyclodextrin and one Brilliant cresy blue ,and the possible geometry of the inclusion complex were discussed.