海巴戟天的离体快速繁殖(简报)

Rapid Propagation of Morinda citrifolia Linn. in vitro

从多果无病虫害的母树中选取的幼嫩侧枝，经表面消毒后切取侧芽并接种到MS基本培养基，附加蔗糖浓度30g/L，BA2mg／L，NAA0．1mg／L初代培养基上，培养40d后，侧芽萌发率达80％以上。增殖培养基与初代培养基相同，以40-50d为一增殖周期，增殖系数达2-3倍。当新芽增殖到足够数量时，切取2-3cm的新芽接种到1／2MS大量元素，全量微量元素，蔗糖浓度为30g/L，IBA1mg／L，活性炭1g/L的生根培养基生根培养基上，生根率高达100％，植株在沙床的移栽成活率均达95％以上。30cm高的植株便可种植到大田。

Young branches from the mother tree of Morinda citrifolia Linn. that bears plenty of fruits without pest and disease were collected as study material. The axillary buds from the branches were cut as the explants and inoculated onto the MS medium supplemented with BA 2 mg/L, NAA 0. 1 mg/L and sucrose 30 g/L. The explants were then cultured for 40 days, and over 80 % of axillary buds were burgeoned. The subculture medium was the same as the first medium. A cycle of multiplication was 40-50days, and the multiplication rate was 2-3 times. The new shoots 2-3 cm high were cut and inoculated onto the rooting medium which was 1/2MS macro-element, total MS micro-element, IBA mg/L, sucrose 30 g/L and active carbon 1 mg/L. The rooting rate was 100 % and over 95 % of plants survived after transplanted onto the sand bed. The young plants 30 cm high were planted to the field.