不同浓度的激素组合对猕猴桃愈伤组织诱导及芽分化的影响

The Effect of Hormone Combination with Different Concentrations on the Callus Induction and Bud Differentiation of Kiwifruit

[目的]寻求代替ZT提高猕猴桃愈伤组织诱导及芽分化的最佳激素组合。[方法]以美味猕猴桃离体茎段为外植体,以MS为基本培养基,附加2,4-D0.5、1、2.0mg/L和NAA0.1、0.3、0.5mg/L与6-BA0.5mg/L分别组成诱导愈伤组织的6个激素组合,并以同样浓度的2,4-D和NAA与6-BA1.0mg/L分别组成分化芽的6个组合激素,研究不同浓度的激素组合对猕猴桃愈伤组织诱导及芽分化的影响。[结果]6种不同浓度的激素组合对猕猴桃茎段愈伤组织均有一定的诱导作用,其中2,4-D1.0mg/L+6-BA0.5mg/L的组合有利于愈伤组织诱导,诱导率达86.1%;绿色愈伤组织在MS培养基附加激素2,4-D+6-BA组合中芽的分化率很低,而在附加NAA+6-BA组合中芽的分化率较高,其中,NAA0.1mg/L+6-BA1.0mg/L的组合有利于芽分化,芽分化率为75.2%。[结论]2,4-D与6-BA组合对猕猴桃绿色愈伤组织的芽分化有一定抑制作用。

[Objective] The purpose of study was to seek optimum hormone combination that could replace ZT and increase callus induction and bud differentiation of Actinidia deliciosa.[Method] With the stem segments of A.deliciosa as explants and MS as basic medium,6 hormone combinations of callus induction in which 0.5,1,2.0 mg/L 2,4-D and 0.1,0.3,0.5 mg/L NAA were matched resp.with 0.5 mg/L 6-BA and 6 hormone combinations of bud differentiation in which 2,4-D and NAA with same concentrations were matched resp.with 1.0 mg/L 6-BA were set up to study the effect of hormone combinations with different concentrations on the callus induction and bud differentiation of A.deliciosa.[Result] 6 hormone combinations with different concentrations had some callus induction on A.deliciosa stem segments,in which,the combination of MS＋2,4-D 2.0 mg/L＋6-BA 0.5 mg/L was in favor of callus induction,with the callus induction rate reaching 86.1%.The green callus had lower bud differentiation in MS medium with hormone combinations of 2,4 -D＋6-BA,but had higher bud differentiation in MS medium with hormone combinations of NAA ＋6-BA,in which,the combination of NAA 0.1 mg/L＋6-BA 1.0 mg/L was in favor of bud differentiation,with the bud differentiation rate being 75.2%.[Conclusion] The hormone combinations of 2,4-D ＋6-BA had some restraining effect on bud differentiation of green A.deliciosa callus.