血管性痴呆小鼠海马ERK_1及ERK_2的表达特征

Expression features of ERK_1 and ERK_2 in CAl area of hippocampus of mice with vascular dementia

目的观察血管性痴呆(VD)小鼠海马神经元中细胞外信号调节激酶1、2(ERK_1、ERK_2)的表达特征,探讨其在VD发病中的作用机制。方法采用双侧颈总动脉反复缺血—再灌注法制备小鼠VD模型,设立假手术组作为对照。术后第29、30天,经跳台试验和水迷宫试验对2组小鼠进行行为学成绩测试,用免疫组化方法观察2组小鼠海马神经元中ERK的表达变化。结果VD模型小鼠学习、记忆成绩较假手术组显著下降(P<0.05)。模型组小鼠海马CA1区ERK_1、ERK_2的表达较假手术组湿著减少(P<0.05)。结论海马神经元内ERK表达减少可能参与了VD的发病机制,应用能促进ERK表达的药物可能成为治疗VD的有效药物之一。

Objective To observe the expression characteristics of extracellular signal-regulated kinase 1,2 （ERK1 and ERK2 ）in the hippocampal neurons of mice with vascular dementia（VD）, and to explore its role in the pathogenesis of VD. Methods The VD models of mice were established by 3-time ischemia/reperfusion in bilateral common carotid arteries. Sham operation group was set up as control group. The behavioral scores in both groups were recorded by step-down test and water maze test at the 29th and 30th day respectively. The expressing features of ERK1 and ERK2 in CA1 area of hippocampus of mice in both groups were observed by immunohisochemistry method. Results The grades of learning and memory of VD mice were obviously decreased,as compared with those of sham operation group（ P 〈 0.05） .The expression of ERK1 and ERK2 in CA1 area of hippocampns in model group was significantly lower than that of sham operation group （ P 〈 0.05）. Conclusion The decrease of expression of ERK1 and ERK2 may participate in the pathogenesis of VD. It is suggested that the application of the drug that could increase the levels of ERK in hippecampus may become one of the effective drugs to treat VD.