共聚物微泡造影剂的制备与体外二次谐波特性研究

Aolymer-encapsulated microbubble contrast agent:preparation and in vitro echogenic characteristics

目的用PLLA-PEG-PLLA共聚物为外壳材料制备微泡造影剂,建立体外声学性质测定装置和方法。方法用开环聚合方法合成三嵌段共聚物;用双乳化法制备空心造影剂微泡;使用光学显微镜和扫描电镜观察形貌;测定微泡的粒径分布;体外测试微泡的二次谐波特性。结果获得一系列在生理盐水中分散性很好的空心微泡,在低机械指数下呈现很好的二次谐波增强信号增强。结论以共聚物PLLA-PEG-PLLA为外壳材料,采用双乳化法得到的空心微泡可以用作为超声造影剂。

Objective To prepare an ultrasound microbubble contrast agent using PLLA-PEG-PLLA copolymer as the shell material, and test their acoustic characteristics in vitro. Methods PLLA-PEG-PLLA tri-block co-polymers were synthesized by ring-opening polymerization. Microbubbles were prepared by using double emulsion technique. Light microscope and scanning electron microscope were applied to observe the morphology of the microbubbles. Their size distribution was analyzed using MASTERSIZER 2000. The acoustic characteristics of microbubbles were tested by using color Doppler Ultrasonography. Results Microbubbles were well dispersed in water. Contrast enhancement was stronger and longer lasting at low mechanical index. Conclusion The polymer-encapsulated microbubbles prepared by using double emulsion technique with PLLA-PEG-PLLA as the shell material can be used as an ultrasound contrast agent