清脂软胶囊对实验性高脂模型大鼠脂肪肝变性程度及对血脂水平的影响

Effect of Qingzhi soft capsule on blood lipid level and pathology of the fatty liver in hyperlipidemic rats

目的观察清脂软胶囊对高脂模型大鼠脂肪肝的病变程度及对血脂水平的影响。方法SD大鼠灌胃高胆固醇脂肪乳剂以制备大鼠高血脂模型,以清脂软胶囊为治疗组,血脂康为阳性对照组,观察实验动物治疗3周后血清总胆固醇、总甘油三酯、高、低密度脂蛋白水平及动脉粥样硬化指数,同时显微镜下观察肝脏脂肪变性程度,进行统计学分析。结果与模型对照组比较,清脂软胶囊二个剂量组(150、75mg/kg)对高脂模型大鼠血清总胆固醇、总甘油三酯水平具有显著的降低作用(P<0.05);对高密度脂蛋白有一定的升高作用,但无显著性差异(P>0.05);大剂量组有降低低密度脂蛋白的作用;同时,清脂软胶囊组动脉硬化指数显著低于模型对照组(P<0.01)。肝脏病理结果显示,清脂软胶囊可以减轻高脂模型大鼠肝脏脂肪病变的程度。结论清脂软胶囊可以调节高脂模型大鼠血脂水平,降低动脉硬化指数,减轻模型大鼠脂肪肝的病变程度。

Objective To observe the effects of Qingzhi soft capsule, a preparation of traditional Chinese medicine, on blood lipid level and the pathology of fatty degeneration of the liver in rats with hyperlipidemia. Methods SD rats were subjected to daily intragastric administration of a high-cholesterol emulsion （10 ml/kg） every morning to induce hyperlipidemia. The rats with established hyperlipidemia were then randomized into 4 groups and received every afternoon intragastric administration of high-dose （150 mg/kg） and low-dose （75 mg/kg） Qingzhi capsule, Xuezhikang （150 mg/kg, positive control ）, and distilled water of the same volume （model group）, respectively. A normal control group was also used in which the rats were given only distilled water in the same manner. After 21 days of treatmant, all the rats were sacrificed for determining the serum total cholesterol （TC）, triglyceride （TG）, high-density lipoprotein cholesterol （HDL-C）, and low-density lipoprotein cholesterol （LDL-C） levels as well as the atherosclerosis index （AI）. The liver of the rats was taken for examination of the pathology of fatty degeneration under microscope. Results The TC and TG levels in both of the Qingzhi capsule groups were significantly lower than those in the hyperlipidemic model group, but no significant differences were noted in HDL-C and LDL-C levels between the Qingzhi and model groups. Al was markedly lower in the two Qingzhi groups than in the model group. Pathological examination of the liver showed milder hepatic pathology of fatty degeneration in the Qingzhi groups than in the model group. Conclusion Qingzhi soft capsule can modulate the blood lipid levels, ameliorate the hepatic pathology of fatty degeneration and lowers AI in in hyperlipidemic rats.