Trophinin在人卵母细胞及着床前胚胎细胞上的表达与意义

Expression of trophinin in human oocytes and preimplantation embryos

目的研究trophinin在人卵母细胞、着床前胚胎细胞上的表达与意义。方法采用间接免疫荧光技术、应用激光扫描共聚焦显微镜对9个卵母细胞、16个分裂胚胎及12个囊胚进行了荧光检测。结果trophinin蛋白在人卵母细胞、分裂胚胎以及囊胚上均有表达,且表达逐渐显著增强(P<0.05)。结论人囊胚可能通过trophinin与表达有相同粘附分子的着床窗期子宫内膜发生同种粘附,从而在人胚胎着床过程中起重要作用。

Objective To investigate the significance of trophinin expression in human oocytes and preimplantation embryos. Methods The expression of trophinin in 9 human oocytes, 16 blastomeres and 12 blastocysts were detected by indirect immunofluorescence assay and observed under laser scanning confocal microscope. Results Human oocytes, blastomeres and blastocysts were all positive for trophinin expression, and the positivity intensified significantly in the course of the embryonic development （P〈0.05）. Conclusions Trophinin may play an important role in human embryo implantation by mediating homophilic adhesion between the embryo and the endometrium during the implantation window.