乙肝患者MBL EXON I 54位基因频率检测

The Study on MBL EXON I 54 Gene Frequency in Hepatitis B Virus Infection

目的:检测健康人及慢性乙肝患者MBL基因Exon I 54位密码子点突变的情况。方法:对MBL 54位密码子基因突变采用PCR-RLFP检测法。结果:检测64例健康汉族人54位密码子的点突变情况,野生型(GGC/GGC)为44例,占68.8%;突变杂合型(GGC/GAC)为19例,占29.6%,突变纯合子型(GAC/GAC)为1例,占1.6%,基因频率分别为GGC为0.836;GAC为0.164。检测54例乙肝患者,野生型为21例,占38.89%;突变杂合型为32例,占59.26%,突变纯合子型为1例,占1.85%,基因频率分别为GGC为0.6852;GAC为0.3148,健康人及慢性乙肝患者MBL基因54位密码子点突变有显著性差异,χ2=10.681P=0.005(按基因型统计);χ2=6.258P=0.012(按基因统计)。结论:乙肝患者组较健康对照组MBL基因Exon I 54位密码子点突变频率显著升高,提示该项指标在乙肝发生机理中的作用应进一步研究。

Objective： To analyze the genotypes of MBL Exon I 54 in the healthy and patients with HBV infection. Methods： The situations of PCR-RFLP method was used to describe and detect genotypes of MBL Exon I 54. Results：The situations of genotypes of MBL Exon I 54 in 64 healthy cases were that 44 people （68. 8%）were wildtype, 19 people（29.6%）were heterozygous（wildtype and mutant）1 people was mutant occupying 1.6 %, the genotypes of GGC and GAC were 0. 836 and 0. 164 respectively. The situations of genotypes of MBL in 54 HBV infections were that 21 people（38.89%） ,32 people（59.26%）were heterozygous（wild-type and mutant）, 1 peoplewas mutant（1.85%） ,the genotype of GGC and GAC were 0. 6852 and 0. 3148. there was statistical significance between HBV infections and healthy（x^2= 10. 681 ,P=0. 005（genotype）,x^2 =6. 258 P= 0. 012（gene））. Conclusion：The results show th.at MBL genotypes influence recovery from hepatitis B virus infection and need to study deeply.