实验性腹膜炎小鼠脾、肾钙调神经磷酸酶的变化

The Alteration of Calcineunn Activity in Spleen and Kidney of Experimental Peritonitis Mice

目的:观察实验性腹膜炎小鼠脾、肾钙调神经磷酸酶(CaN)在腹膜炎病程中变化。方法:36只小鼠随机分为6组,即腹膜炎48h、5d、10d、14d、21d组与对照组,腹膜炎组腹腔注射1%冰醋酸0.2mL,对照组腹腔注射等量生理盐水,每组各6只。采用比色法测定脾、肾组织CaN活性。结果:脾CaN活性在实验性腹膜炎5d、10d、14d、21d呈持续性低水平(P<0.01);肾CaN活性在实验性腹膜炎10d达高峰14d、21d与10d相比呈下降趋势但仍高于对照组,差异有显著性。结论:实验性腹膜炎小鼠脾、肾CaN活性变化与病程进展有关。

Objective：AIM To investigate the alteration of calcineurin activity in spleen and kidney in experimental peritonitis mice. Methods：36 Kunming mice were randomly divided into 6 groups,which were exerimental Peritonitis groups for 48h,Sd,10d,14d,21d and control group. In the groups of experimental peritonitis, the model of non-specific peritonitis was induced by injecting ice acetate acid into mouse＇s peritoneal cavity,and the same quatity of normal saline was injected to animals in control group. CaN activity in spleen and kidney was measured by using PNPP as substrate. Results： CaN activity in spleen was significantly decreased in experimental peritonitis groups of 5d, 10d, 14d,21d compared with that of control group（P〈0. 01）, while in kidney was increased for the groups of 10d,14d,21d（ P〈0.01or P〈0. 05） compared with that of control group. There was a peak time at 10d. Conclusion： CaN activity might be involved in functional regulation for spleen and kidney in experimental Peritonitis.