摘要
【目的】定位猪CACNA2D1基因并分析该基因是否可作为影响猪某些生产性状的候选基因。【方法】扩增并测定猪CACNA2D1基因的部分序列,运用辐射杂种细胞系对其进行定位并将定位结果与相关遗传图谱进行整合分析。【结果】将猪cAcNA2D1基因定位在猪9号染色体79.3~86.7cM的位置上,发现在cACNA2D1基因定位区域有3个分别影响母猪发情期排卵数、胴体肩胛重以及10周龄体重的QTL,在定位区域附近有影响猪屠宰24h后肌肉pH值的QTL。【结论】猪CACNA2D1基因可作为猪繁殖性状、胴体性状、生长性状甚至肉质性状的候选基因进行进一步的研究。
[Objective] The study was conducted to map porcine CACNA2D1 gene and to analyses if the gene could be used as a candidate gene influencing some production traits of porcine. [Method] Porcine CACNA2D1 gene was mapped by radiation hybrid panel and the mapping result was compared with correl- ative genetic maps after partial sequence of porcine CACNA2D1 gene beening amplified and sequenced. [Result] Porcine CACNA2D1 gene was located on 79.3~86.7 cM of porcine chromosome 9. Three QTL affecting ovulation rate, shoulder weight, body weight at 10 weeks of age respectively have been found in the located domain and one QTL affecting pH 24 hours post mortem has been found also near the area of CACNA2D1 gene. [Conclusion] CACNA2D1 gene could be studied more as a candidate gene influencing reproduction, carcass, and growth traits, even as the meat quality traits of porcine.
出处
《西北农林科技大学学报(自然科学版)》
CSCD
北大核心
2008年第1期49-53,共5页
Journal of Northwest A&F University(Natural Science Edition)
基金
湖北省教育厅优秀中青年人才项目(Q200626001)