抗氧化酶在硝基苯中毒小鼠睾丸细胞凋亡中的作用

Effect of antioxidase on testis cell apoptosis induced by nitrobenzene in mice

将10周龄雄性昆明小鼠随机分为硝基苯染毒组(26 mg/kg、52 mg/kg、105 mg/kg)、花生油溶剂对照组、生理盐水对照组。对各组试验小鼠进行处理后,于第30 d检测小鼠血清和睾丸中SOD、GSH-Px、CAT的活性及MDA含量,并通过DNA Ladder条带和透射电镜检测睾丸细胞的凋亡。结果,染毒组血清及睾丸中SOD、GSH-Px、CAT的活性显著或极显著(P<0.01或P<0.05)低于溶剂对照组,MDA含量显著或极显著(P<0.01或P<0.05)高于溶剂对照组,染毒组均显示DNA Ladder条带;电镜下细胞核皱缩,异染色质边聚,呈现典型凋亡现象。结果表明,硝基苯中毒能够诱使睾丸发生氧化应激,进而导致睾丸细胞发生凋亡,抗氧化酶在睾丸细胞凋亡中具有一定的作用。

Ten week old male Kunming mice were divided randomly into 3 nitrobenzene treated groups （26 mg/kg, 52 mg/kg and 105 mg/kg, respectively）, arachis oil control group and normal saline control group. The activities of SOD, GSH-Px and CAT and the content of MDA in serum and testicle were examined on day 30 post-treatment. Testis cell apoptosis was detected by DNA Ladder and transmission electron microscope. The activities of SOD,GSH-Px and CAT in the 3 nitrobenzene treated groups were＇significantly lower than those in the arachis oil control group,and the contents of MDA in the 3 nitrobenzene treated groups were significantly higher than that in the solvent control group（P〈0.01 or P〈0.05）. DNA Ladder appeared in the 3 nitrobenzene treated groups. Typical apoptosis was observed by the electron microscope. The result showed that nitrobenzene could induce oxidative stress, and lead to the apoptosis of testis cell in male mice,and antioxidase played a great role in testis cell apoptosis of the male mice.