大鼠三叉神经和坐骨神经皮层诱发电位的对比研究

Investigation of comparison between trigeminus evoked potential and nervus ischiadicus evoked potential in normal rat

目的:比较大鼠三叉神经皮层诱发电位(TEP)和坐骨神经皮层诱发电位(IEP)的检测方法、特性及应用价值。方法:取40只Wistar大鼠,分别电刺激一侧第3排第2、3根胡须处皮下及坐骨神经,在对侧硬膜外记录TEP和IEP,将其检测率、波形的稳定性及重复性进行比较。结果:35只大鼠记录到TEP,基本波形为N1-P-N2;25只记录到IEP,基本波形为N-P;TEP的N1-P峰峰波幅明显大于IEP的N-P峰峰波幅(P<0.01),TEP的N1-P峰峰波幅的变异系数明显小于IEP的N-P峰峰波幅,TEP的N1、P峰潜伏期明显小于IEP的N、P峰潜伏期(P<0.01),TEP的N1、P峰潜伏期的变异系数略大于IEP的N、P波。结论:TEP较IEP容易获取,重复性好,波形稳定,灵敏度高,操作简便,损伤小,便于长期观察。

Objective To compare the detection method, characteristic and application value of trigeminus evoked potential （TEP） with nervus isehiadieus evoked potential （IEP） in normal rats. Method Subcutaneous layer of the root of the second, third whisker in the third row and nervus isehiadicus on one side of 40 normal Wistar rats were stimulated by electricity, TEP and IEP were detected from the contralateral epidural surface, and the detection rate, stability and duplication of waveform were compared. Results TEP was detected in 35 rats, and the basic waveform was N1 - P - N2 ; IEP in 25 rats, the basic waveform of N - P. The amplitude of N1 - P in TEP was bigger than N -P in IEP, with significant difference （P 〈0.01 ）, and the amplitude coefficient variation of N1 -P was significantly smaller than N - P in IEP. The latency of N1 - P in TEP was shorter than N - P in IEP, with significant difference （ P 〈 0.01 ）, and the latency coefficient variation of N1 - P in TEP was a little bigger than N - P in IEP. Conclusion The waveform of TEP is easy to attain, and with the advantages of excellent repeatability, stable waveform, high sensitivity, convenient operation and fewer damage compared with IEP.