新型 L-乳酸盐酶电极的研制

Development of a New Enzyme Electrode for L-Lactate Detection

本文研制一种新型乳酸盐酶生物传感器，此传感器以复合酶ＮＡＤ＋与ＬＤＨ共固定在经ＣＮＢｒ活化的琼脂糖上作为敏感膜。用厚为０３ｍｍ的粒状琼脂糖制成的敏感膜电极，其响应性能最佳。此乳酸盐酶电极测试的最优条件是：在ｐＨ８５的Ｔｒｉｓ－ＨＣｌ缓冲液中进行，温度２５℃，此缓冲液中含１５ｍｍｏｌ·Ｌ－１Ｖｋ３电子介体。此电极的线性反应范围为００４～２１μｍｏｌ·Ｌ－１，每μｍｏｌ·Ｌ－１乳酸盐的敏感度为１３７ｎＡ，工作寿命为１９天。已将此电极用于检测血清中乳酸盐含量，得到满意的结果。

A new lactate enzyme biosensor with Co inmobilized LDH and NAD + on CNBr activedagarose as sensing membrane was developed.Sensors formed with grain state sensing membrane of 0 3 mm thickness have better response characteristics than those formed with gel shiced sensing membrane of various thickness.The optimum testing condi tions of the lactate enzyme electrode are as follows 0 025 mol·L -1 Tris HCl buffer at pH8 5 and 25 ℃.The buffer contained 1 5 mmol·L -1 Vi twmik k 3 as electron mediator.The linear range of detection of the sensor is 0 04 to 2 1 μmol·L -1 with sensitivity of 137 nA/μmol·L -1 lactate.The lifetime of the sensor is 19 days.The sensor was also used for tesitng serum samples with satisfactory results