垂丝海棠插穗生根与可溶性蛋白和激素变化的关系

Studies on Changes of Soluble Proteins in the Cuttings Rooting of Malus halliana Roehne

利用SDS-PADE电泳技术和酶联免疫吸附分析法,研究了垂丝海棠插穗切口处的可溶性蛋白质变化和激素变化与生根的关系。结果表明:46KD、39KD、31KD、28KD、26KD、24KD等可溶性蛋白质等与生根有关。其中,39KD、26KD、24KD是调控蛋白,其作用是诱导细胞的脱分化和再分化,愈伤组织形成及根原基的产生有关,当不定根形成后即消失,46KD、31KD、28KD蛋白是伴随不定根产生而新形成的蛋白,促进垂丝海棠插穗的生根,46KD蛋白是一种阻碍细胞分化的蛋白,对生根不利。

By applying the techniques of SDS-PAGE ＆ ELISA, this thesis researched explored the relationship between rooting and the variances of both soluble proteins and endogenous plant hormones in the process of Malus halliana＇s cutting. The experiment results showed that： during the cutting procedure, the soluble proteins of 46KD,39KD,31KD, 28KD,26KD,24KD were related to the rooting. The soluble proteins in 46KD,39KD.31KD,28KD controlled the callus＇ growing and adventitious root primordium differentiation, furthermore, they disappear when the cuttings generated ad- ventitious roots. While the rooting proceeded, some new kinds of soluble proteins, like 46 KD,31 KD.28 KD, which were good for promoting new adventitious roots had been generated. It seemed that the soluble protein 46KD in the cuttings had some disadvantages to the adventitious root primordium differentiation of the cuttings rooting.