摘要
采用香花槐的花器官做外植体,进行组织培养和植株再生系统的研究。结果表明:2,4-D对于香花槐愈伤诱导的启动是必需的,而多种激素配比会使诱导率提高;选择浓度为0.3～0.5mg/L的BA和浓度为0.1mg/L的NAA配合使用,能使花药愈伤达到较高分化率;1/2MS+IBA0.05mg/L和1/2MS+IBA1.0mg/L的诱导组培苗生根效果较好。
Robinia idaho regeneration system and its tissue culture has been studied with explant of different part of its flower. The results show that 2,4-D is indispensable for callus induction of anther, ovary and filament while receptacles and petals cannot be induced in this experiment. Combination of 0. 3-0. 5 mg/L BA and 0. 1 mg/L NAA are effective to differentiate anther callus. 1/2MS medium supplemented with IBA 0. 05mg/L and 1/2MS＋IBA 1.0mg/L can well promote seedings to take roots.
出处
《北方园艺》
CAS
北大核心
2008年第1期191-193,共3页
Northern Horticulture
基金
云南省农业科技攻关资助项目(2006NG34)
关键词
香花槐
花药培养
再生植株
愈伤组织
Robinia idaho ,Anther culture
Plant regeneration
Ballus introduction
