焦炉工人血浆BPDE-白蛋白加合物与外周血淋巴细胞DNA损伤水平的相关关系

Relationship between Plasma BPDE-Albumin Adduct and DNA Damage of Peripheral Blood Lymphocytes among Coke Oven Workers

目的探讨焦炉工人早期生物效应标志物DNA损伤与生物有效剂量标志物苯并(a)芘二氢二醇环氧化物(BPDE)-白蛋白加合物之间的关系。方法选取某焦化厂207名焦炉工人作为接触组,102名非焦炉作业人员为对照组,采用反相高效液相色谱法测定血浆中BPDE-白蛋白加合物浓度,采用彗星实验测定外周血淋巴细胞DNA损伤水平。结果焦炉工人的血浆BPDE-白蛋白加合物浓度和外周血DNA损伤水平明显高于对照组(P<0.01)。使用多元logistic回归分析校正了个体的工龄、吸烟和饮酒状况后,焦炉工人BPDE-白蛋白加合物浓度增高的危险度是对照组的1.72倍(P<0.05),焦炉工人DNA损伤水平增高的危险度是对照组的1.96倍(P<0.05)。BPDE-白蛋白加合物与DNA损伤水平在焦炉工人中存在明显的正相关关系(Spearman偏相关系数=0.235,P<0.01),但在对照组中不存在相关关系(Spearman偏相关系数=0.093,P>0.05)。结论焦炉工人生物有效剂量标志物BPDE-白蛋白加合物和早期生物效应标志物DNA损伤之间存在明显相关。

Objective To explore the relationship between the DNA damage of biomarkers for early biological effects in coke oven workers and BPDE-AIb adducts. Methods 207 male coke oven workers were selected as the exposed group and 102 unexposed to coke oven as the controls. Reverse-phase highperformance liquid chromatography was used to measure BPDE-AIb adduct concentrations in plasma and alkaline comet assay for DNA damage in peripheral blood lymphocytes. Results The concentration of BPDE- Alb adducts and the Olive tail moment（Olive TM）in exposed group were significantly higher than those in control group（P〈0.05）. Multivariate logistic regression analysis revealed that the odds ratio（OR）for BPDE- Alb adduct and Olive TM associated with the exposure were 1.72 and 1.96 respectively. Results showed that there was significant correlation between the concentrations of BPDE-AIb adduct and Olive TM levels in exposed group（r=0.235,P〈0.01）but not in the control group（r=0.093,P〉0.05）. Conclusions The relationship between biomarkers of biological effective and early biological effects was significant among coke oven workers.