加减玉女煎抗心室重构的实验研究

Experimental research of Jiajian Yunü Decoction on ventricular remodeling

目的:探讨经典复方加减玉女煎抗心室重构(ventricular remodeling,VR)的作用及作用机制。方法:应用左旋-甲状腺素(L-thyroxine,L-Thy)、腹主动脉不完全结扎法(abdominal aorta banding,AAB)致大鼠心室重构等两种动物模型,观察加减玉女煎对心脏指数(heart index,HI)、血清一氧化氮(nitric oxide,NO)、心肌血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)、内皮素1(endothelin-1,ET-1)等神经内分泌因子及心肌羟脯氨酸(hydroxyproline,Hyp)含量、谷胱甘肽-还原酶(glutathioneperoxidase,GSH-Px)、超氧化物岐化酶(superoxide dismutase,SOD)活性的影响,图象分析法观察心肌细胞横断面积(car-diomyocyte cross section area)。结果:与模型组相比,加减玉女煎可显著降低动物HI,升高血清NO浓度,降低心肌AngⅡ、ET-1、Hyp含量,升高心肌GSH-Px、SOD活性,减小心肌细胞横断面积。结论:复方加减玉女煎对心室重构具有明显的改善作用。

AIM： To explore the effects and mechanisms of classic prescription Jiajian Yunü Decoction （JJYND） on ventricular remodeling. METHODS ： Two kinds of ventricular remodeling model were induced by injecting L-thyroxine intraperitoneally or banding abdominal aorta in rats. The effects of JJYND on heart index （HI） and concentrations of neuroendocrine factors such as serum nitric oxide （ NO）, myocardium angiotensin Ⅱ（AngⅡ）, endothelin-1 （ ET-1 ）, activities of glutathione peroxidase （ GSH-Px）, superoxide dismutase （ SOD）, concentration of myocardium hydroxyproline （Hyp）, cross section area of cardiomyocyte were determined. RESULTS： JJYND could significantly reduce the heart index, cardiomyocyte cross section area, elevate serum NO concentration, restrain myocardium AngⅡ, ET-1, Hyp concentrations and improve myocardium GSH-Px, SOD activities. CONCLUSION： Prescription JJYND has good effects on attenuating ventricular remodeling.