灯盏花素分散片溶出度测定方法的研究

Determination of the dissolution of Breviscapinun Dispersive Tablet by HPLC

目的：建立灯盏花素分散片中野黄芩苷的溶出度测定方法。方法：以磷酸盐缓冲液（pH7．4）为介质，选择小杯法（2005版药典二部附录XC溶出度测定法第三法）操作；用HPLC法检测，计算灯盏花素分散片溶出度。结果：本法平均回收率为101．93％。RSD为1．09％。在0．007～0．180g／mL范围内浓度和峰面积呈良好线性，r=0．9999；本品受转速影响小，受溶剂影响大。结论：本方法具有灵敏、准确、快速的优点可用于灯盏花素分散片的质量控制。

AIM： To establish a method for determing the dissolution of Breviscapin Dispersive Tablet. METHODS： Breviscapin release was investigated in phosphate BS（pH7.4） by the third method for the release determination （ Chinese Pharmacopoeia 2005 edition）. Breviscapin content was assayed by HPLC and the release percentage was then calculated. RESULTS： The average recovery of scutellarin was 101.93% ,RSD = 1.09%. Good linear relationship was shown in the concentration range of 0. 007 -0.18 mg/mL, r =0. 999 9. The dissolution was almost unaffected by rotation rate, but greatly affected by the type of solvent. CONCLUSION ： The method is sensitive,accurate and quick for the quality control of Breviscapin Dispersive Tablet.