冠心病患者外周血内皮祖细胞数量与心血管危险因素的关系

Relationship between the number of circulating endothelial progenitor cells and cardiovascular risk factors in patients with coronary heart disease

目的:观察冠心病患者外周血内皮祖细胞(EPCs)数量与心血管危险因素之间的关系。方法:选择20例冠心病患者(G1组,n=20)和10例非冠心病患者(G2组,n=10),分别抽取外周血进行EPCs的分离培养,于第10天对EPCs进行鉴定,并于倒置相差显微镜下计数内皮祖细胞克隆形成单位(EPC-CFU),评估外周血EPCs水平。分析外周血EPCs数量与心血管的各个危险因素(高血压、糖尿病、血清低密度脂蛋白(LDL)、总胆固醇、高敏C反应蛋白(hsCRP)水平)及整体危险性(用Framingham危险积分定量评估)的关系。结果:G1组外周血EPCs数量较G2组明显减少(10.3±2.5vs17.7±2.2),差异有统计学意义(P<0.05)。冠心病患者外周血EPCs数量与Fram-ingham危险积分、高血压、血清LDL、hsCRP水平呈负相关。结论:冠心病患者外周血EPCs数量减少,并与Framing-ham危险积分呈负相关。

Aim： To investigate the relationship between the number of circulating endothelial progenitor cells（EPCs） and cardiovascular risk factors in patients with coronary heart disease （CHD）. Methods：Twenty CHD patients （G1 group） and 10 matched control subjects （G2 group） were selected in this study. Peripheral blood samples were drawn to isolate and culture EPCs, respectively. The circulating EPCs were characterized as adherent cells double positive for acLDL-DiLuptake and FITC-UEA-I binding by direct fluorescent staining under a laser scanning confocal microscope. EPCs populations were assessed using the colony forming unit assay （EPC-CFU） through an inverted phase contrast microscope after 10 days culture. The relationship between EPCs populations and each cardiovascular risk factors （including hypertension,diabetes,low density lipoprotein, total cholesterol and hsCRP）and integrating risk （assessment with the Framingham risk score） were analyzed. Results： The number of EPCs in G1 group was lower than that in G2 group（ 10.3 ± 2.5 vs 17.7 ± 2.2 ,P 〈 0.05 ）. The number of circulating EPCs has a negative correlation with the Framingham risk score （ r = - 0. 462,P 〈 0.05 ） and the levels of blood pressure,serum LDL and hsCRP. Conclusion： Circulating EPCs populations are lower in CHD patients and has a negative correlation with the Framingham risk score.