胃癌组织中多巴胺受体亚型D_4和c-fos mRNA的表达

Expression of dopamine receptor subtype D_4 and c-fos mRNA in human gastric cancer tissue

目的:检测胃癌组织中多巴胺受体亚型D4和癌基因c-fos mRNA的表达,探讨2者在胃癌发生、发展中的作用。方法:应用原位杂交技术检测42例胃癌和35例正常胃黏膜组织中的D4和c-fos mRNA的表达,并分析2者与临床病理指标的关系及2者的相关性。结果:与正常胃黏膜比较,胃癌组织中D4阳性率明显降低(P<0.01),c-fos阳性率明显升高(P<0.01)。D4在肿瘤直径≥5cm组中的阳性率明显低于<5cm组(P<0.05),但与肿瘤分化程度、浸润深度及有无淋巴结转移无关(P>0.05);c-fos在高中分化及肿瘤直径≥5cm胃癌组中的阳性率分别高于低分化组(P<0.01)和<5cm组(P<0.05),而与肿瘤浸润深度及有无淋巴结转移无关(P>0.05);胃癌组织中D4和c-fos的表达呈负相关(P<0.05),D4表达阳性的胃癌组织中c-fos阳性率明显低于D4阴性组。结论:D4和c-fos在胃癌发生及癌细胞增殖中起重要作用,且c-fos与胃癌分化密切相关。胃癌组织中D4低表达与c-fos高表达可能协同参与了胃癌的发生发展。

Aim ： To detect the mRNA expression of dopamine receptor subtype D4 and c-fos oncogene in human gastric cancer tissue, and investigate their role in the occurrence and development of gastric cancer. Mothods：The mRNA expression of D4 and c-fos in 42 cases of gastric adenocareinoma and 35 cases of normal stomach tissue was detected by in situ hybridization; their relations with each other and with the clinical pathological parameters of gastric cancer were also investigated. Results ： Compared with normal stomach tissue,the mRNA expression of D4 was decreased and that of c-fos was increased in gastric cancer tissue, respectively （P 〈 0.01 ）. The two parameters were both associated with the size of tumor （P 〈 0.05 ）, but not with the invasive depth or lymph-node metastasis of gastric cancer（ P 〉 0.05 ）. And c-fos mRNA was also associated with the differentiation of gastric： adenocarcinoma（P 〈 0.01 ）. The mRNA expression of D4 was negatively correlated with that of c-fos（P 〈 0.05 ）. Conclusion ：The expression of c-fos mRNA is associated with the differentiation of gastric cancer. The decreased expression of D4 and increased expression of c-fos in gastric cancer may cooperate with each other in the occurrence of gastric cancer.