摘要
目的:制备人B7-1(hB7-1)转基因肝癌瘤苗。方法:用逆转录病毒转移系统将hB7-1基因导入人肝癌细胞株HepG2,并用RT-PCR、PCR-Southern杂交法检测转染空载体pLXSN的HepG2/neo细胞和转染重组质粒pLX-SN/hB7-1的HepG2/hB7-l细胞中hB7-1mRNA表达;间接免疫荧光法检测HepG2细胞、HepG2/neo细胞、HepG2/hB7-1细胞形态,计算当日细胞绝对数,绘制生长曲线。结果:所建立的肝癌瘤苗(HepG2/hB7-1)细胞可高效表达hB7-1分子。基因转染对HepG2细胞的生长形态及生长曲线无明显影响。结论:逆转录病毒基因转移系统是使肝癌细胞高效表达hB7-1分子的有效途径。
Aim： To prepare hepatocellular carcinoma vaccine transfected by hB7-1 gene. Methods：The hB7-1 gene was transfered into HCC cell line HepG2 by the retrovirai vector delivery system. The methods of reverse transcription polymerase chain reaction （RT-PCR） , Southern blot, indirect immunofluorescent microscope and flow cytometric assay were used to analyze the expression levels of hB7-1 in HepG2/hB7-1 cells. Results： The established hepatocellular carcinoma vaccine could effectively express hB7-1 molecule. The gene transfection did not influence the growth shape and curve of HepG2. Conclusion ： The retroviral vector delivery system is an effective method which can achieve the high-expression of hBT-1 in HCC cells.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2008年第1期125-127,共3页
Journal of Zhengzhou University(Medical Sciences)
关键词
共刺激分子
B7-1
基因治疗
肝癌
costimulatory molecule
B7-1
gene therapy
hepatocellular carcinoma
