摘要
目的:探讨大鼠急性心肌梗死后心肌组织中AngⅡ、ERK1/2、c-fos mRNA含量的变化及培哚普利的干预作用。方法:结扎大鼠左冠状动脉前降支建立心肌梗死模型,随机分为心肌梗死组(A组,n=14)和培哚普利干预组(B组,n=14),另设假手术组为对照(C组,n=14)。术后24hB组给予培哚普利灌胃,A、C组给予蒸馏水灌胃,6周后处死,取心脏并计算左心室质量指数;分别采用放射免疫法、免疫组织化学法、实时荧光定量PCR法测定各组大鼠非梗死区心肌组织中AngⅡ含量、ERK1/2含量及原癌基因c-fos mRNA的含量。结果:6周后A、B组左心室质量指数、AngⅡ、ERK1/2及c-fos mRNA含量均较C组明显升高(P均<0.05);与A组相比,B组左心室质量指数、AngⅡ、ERK1/2及c-fos mRNA含量均明显下降(P均<0.05)。结论:培哚普利能降低AngⅡ,ERK1/2,c-fos mRNA的表达,改善左心室重塑。
Aim : To study the effects of perindopril on the content of Ang Ⅱ ,ERK 1/2 and c-fos mRNA in myocardial tissue after acute myocardial infarction in rats. Methods:After the model of myocardial infarction was established by ligating the left anterior descending artery of rats, the survivals were randomly divided into two groups : group A ( myocardial infarction group, n = 14) and group B (perindopril group, n = 14). Meanwhile, the other 14 sham-operated rats were used as control group( group C, n = 14). Each group was treated with direct gastric gavage from the second day, and rats of group B were treated with perindopril, group A and C ,with pure water. Six weeks later,the heart was havested, and the left ventricular weight index was calculated, the Ang Ⅱ content, the ERK1/2 content and the expression of c-fos mRNA were respectively detected with radioimmunoassay, immunohistocbemistry method and real-time fluorescent quantitative PCR. Resulits: Compared with group C, Ang Ⅱ , ERK1/2 content, expression of c-fos mRNA and left ventricular weight index significantly increased in group A and B (P 〈0.05 ). The above parameters in group B were lower than those in group A( P 〈 0.05). Conclusion:Perindopril can remarkably reduce the expression of c-fos mRNA and the content of Ang Ⅱ and ERK1/2 in myocardium,thus improve the ventricalar remodeling.
出处
《郑州大学学报(医学版)》
CAS
北大核心
2008年第1期146-149,共4页
Journal of Zhengzhou University(Medical Sciences)
关键词
心肌梗死
心室重塑
培哚普利
信号转导
大鼠
myocardial infarction
ventricular remodeling
perindopril
singal transduction
rat