固体脂质纳米粒中β-榄香烯含量的气相色谱法测定

Determination of β-elemene in β-elemene solid lipid nanoparticles by gas chromatography

目的：建立β-榄香烯固体脂质纳米粒中β-榄香烯的气相色谱测定方法。方法：以质量分数10％PEG-20M（2m×2．6mm）为固定相，60～80目的Shimalite W（NAW）为担体，注射部位温度为200℃；FID检测温度为200℃，柱温150℃，N2为载气，流速50mL／min，内标为萘。结果：β-榄香烯的检测不受自身杂质及辅料的影响，在3．75～75．0mg／L范围内，线性关系良好（r=0．9992），回收率大于99％。结论：本法简单、准确，可用于β-榄香烯固体脂质纳米粒中β-榄香烯的含量测定。

Aim ： To develop a gas chromatography for the determination of β-elemene in β-elemene solid lipid nanoparticles. Methods：10% PEG-20M was used as stationary phase （2 m × 2.6 mm）,Shimalite W（ NAW,60～ 80 meshes） as supporter and naphthalene as internal standard. The temperature of injection port, FID detector anti column oven were set at 200 ℃ ,200 ℃ and 150 ℃ ,respectively. N2 was used as carrier gas with a column flow rate of 50 mL/min. Results： Under the chromatographic condition, β-elemene could be well separated from other components. The calibration curve was linear over the range of 3.75 ～ 75.0 mg/L （ r = 0. 999 2） and the average recovery was higher than 99%. Conclusion ： This method is simple and accurate in detecting β-elemene in β-elemene solid lipid nanoparticles.