反相高效液相色谱法测定帕马溴原料及氨酚帕马溴片中帕马溴含量

Determination of pamabrom in pamabrom and pamabrom paracetamol tablets using RP-HPLC

目的：建立测定帕马溴原料及氨酚帕马溴片中帕马溴含量的反相高效液相色谱法（RP-HPLC）。方法：采用RP-HPLC，Diamonsil C18色谱柱（5μm，150mm×4．6mm），流动相为0.005mol／L磷酸二氢铵溶液-甲醇（65：25）。检测波长为280nm，流速1．0mL·min^-1。结果：帕马溴线性范围为4．98～79．7mg／L（，=0．999），平均回收率为100．4％，RSD为0．96％。结论：该方法简便、准确、灵敏，适用于帕马溴的含量测定。

Aim：To establish a method of the opposition highly effective liquid phase chromatography （RP-HPLC） to examine pamabrom content in the pamabrom raw material and acetaminophen and pamabrom tablets. Methods： RP-HPLC was adapted, on a column of Diamonsil C18（5 μm, 150 mm × 4.6 mm） ,using the mobile phase of 0. 005 mol/L NH4H2PO4- methanol （65 ： 25）, at a flow rate of 1.0 mL/min and the detection wavelength of 280 nm. Results： The calibration curve of pamabrom was linear over the range of 4.98 ～ 79.7 mg/L （ r = 0. 999）. The average recovery was 100.4% and RSD was 0.96%. Conclusion： The method is simple, accurate and sensible, and is suitable for the determination of pamabrom.