知母中总皂苷提取工艺的优化

Optimization of the extraction technology for the total hydrolysate in timosaponin

目的：考察知母中总皂苷的提取工艺。方法：以知母中菝葜皂苷元为定量检测指标，HPLC法测定菝葜皂苷元的含量；通过正交实验考察了3个因素（提取溶剂的浓度，固液体积比和提取时间）对知母中总皂苷提取率的影响。结果：标准曲线线性范围为0．10～1．10g／L（r=0．9998），平均回收率（98．00±3．43）％。采用索氏提取法筛选的知母中总皂苷的最佳提取丁艺为：提取剂为体积分数50％的乙醇，固液体积比为1：10，提取时间为4h。结论：所确定的知母总皂苷的提取工艺合理可行，值得进一步向工业化生产推广。

Aim： To investigate the extract technology for hydrolysate in timosaponin. Methods：The orthogonal test with three levels of three factors：the extracting time, the solid/liquid ratio and concentration of solvent, using hydrolysate as indexes to evaluate the results. Results： The calibration curve was linear over the range of 0. 10 ～ 1. 10 g/L （r = 0. 999 8 ）. The average recovery was （ 98.00 ± 3.43 ） % . The optimal extracting conditions for hydrolysate were 50% ethanol, and solid/liquid ratio of 1 ： 10 and extracting time of 4 h. Conclusion ：The extract technology for hydrolysate in timosaponin is feasible and reasonable.