摘要
根据大豆磷脂的理化性质,采用溶剂萃取法与柱色谱法相结合的工艺制备高纯大豆卵磷脂(PC),研究了各个工艺参数对提取得率与纯度的影响。实验结果表明:大豆粉末磷脂经95%乙醇萃取后进行柱色谱分离,可获得大量纯度>90%的卵磷脂。萃取最佳条件为:萃取温度50℃、萃取时间30min、萃取固液比1:8,实验所用柱色谱规格:￠40mm×300mm,其最大上样量为30g,最佳流速为20mL/min。
Purified Lecithins were produced by solvent extraction and column chromatography according to the propcrties of soybean.The main point and Parameters of the production technology were studied. High purified pc products were obtained from phospholipid powder by theprocedures as： the extraction solvent is ethanol, the extraction time is 30 min,extraction temperature is 50℃ ,m（phospholipid） ：m（solvent） = 1：8 and with ￠ 40 mm × 30 mm chromatographic column separate,the largest sample volumn is 30 g, the best velocity is 20 mL/min.
出处
《大豆通报》
2008年第1期22-25,共4页
Soybean Bulletin
关键词
粉末磷脂
卵磷脂
柱色谱
制备
Powder phospholipid
Leeithin
Column chromatogra-phy
Separation
