茶多糖对四氯化碳致小鼠肝损伤的保护作用

Protective Effect of Tea Polysaccharides on Carbon Tetrachloride Induced Liver Injury in Mice

从六安炒青水提物中分离纯化得到茶多糖TPS-II,对其纯度和分子量进行检测,通过测定小鼠血清超氧化物歧化酶(SOD)活性、天冬氨酸转氨酶(AST)活性、丙二醛(MDA)含量及肝脏指数,研究其对四氯化碳(CCl4)引起的急性肝损伤的保护作用。结果表明TPS-II相对分子量约为1.01×105,总糖含量为85.30%,蛋白含量为2.80%,并对CCl4引起的小鼠急性肝损伤有明显的保护作用。

A polysaccharide named TPS-II was isolated from the water extraction of Liu＇an Roasted Green Tea and purified by chromatography, The homogeneity of TPS-II was identified, its protective effect on carbon tetrachloride induced liver injury in mice was investigated. Results indicated that the molecular weight of TPS-II is about 1,01xl0^5, whose total content of saccharide and protein were 85,30% and 2.80% respectively, and its protective effect against CO14 induced acute liver injury is significant.