L-精氨酸对猪冠状动脉内皮功能的影响

Studies on Effect and its Mechanism of L arginine on Porcine Coronary Artery Endothelium

以氧化低密度脂蛋白（ｏｘｉｄｉｚｅｄｌｏｗ－ｄｅｎｓｉｔｙｌｉｐｏｐｒｏｔｅｉｎ，ｏｘ－ＬＤＬ）诱导猪冠状动脉内皮功能障碍，观察Ｌ－精氨酸（Ｌ－ａｒｇｉｎｉｎｅ，Ｌ－ａｒｇ）对正常及内皮功能障碍的冠脉的影响。结果显示ｏｘ－ＬＤＬ可使猪冠脉舒张功能下降而Ｌ－ａｒｇ可部分阻止这一作用。用逆转录ＰＣＲ技术检测了冠脉血管灌流后结构型一氧化氮合成酶（ｃｏｎｓｔｉｔｕｔｉｖｅｎｉｔｒｉｅｃｏｘｉｄｅｓｙｎｔｈａｓｅ，ｃＮＯＳ）ｍＲＮＡ表达，结果表明ｏｘ－ＬＤＬ可抑制血管ｃＮＯＳｍＲＮＡ表达而Ｌ－ａｒｇ可拮抗这一抑制作用。

We studied the effect of L arginine on normal and abnormal endothelium induced by oxidized low density lipoprotein (ox LDL) in porcine.The results showed that ox LDL (300μg/ml) inhibited endothelium dependent relaxtion (EDR) of porcine coronary arteries and the effect of ox LDL was partially prevented by preincubation with L arginine (3×10 -4 mol/L) for 10 minutes.Reverse transcription RCR showed that constitutive nitric oxide(cNOS) mRNA expression was inhibited by ox LDL,which can be reversed by L arginine indicating that L arginine can improve EDR by upregulationg expression of cNOS.