急性早幼粒细胞白血病的形态学、免疫学和细胞遗传学检测的临床意义

THE CLINICAL SIGNIFICANCE OF THE MORPHOLOGIC,IMMUNOLOGIC AND CYTOGENETIC (MIC) ANALYSIS ON ACUTE PROMYELOCYTIC LEUKEMIA

目的：探讨形态学、免疫学和细胞遗传学（MIC）联合检测对急性早幼粒细胞白血病（M3）的临床意义。方法：将MIC技术用于35例M3患者的诊断、分型及预后评价。结果：形态学检查28例M3a中2例和7例M3v中3例曾误诊为其它白血病，经核型分析确诊。免疫分型10例，多为HLA-DR（－）、CD14（-）、CD33（＋）和CD13（＋）。骨髓染色体分析，正常3例（3／35），31例（31／35）有t（15；17），1例（1／35）具有变异易位．可评价的21例中19例（19／21）达到CR，具有复杂枝型和变异易位各1例未能取得缓解。结论：MIC联合检测在M3的诊断、分型、评价预后等有重要价值。

Obective: Studying the clinical significance of MIC analysis on acute promyelocytic leukemia(M3).Methods: Using MIC techniques. Results: 2 of 28 cases with M3. and 3 of 7 cases with M3v were mistaken forother types of acute leukemia when 35 cases were diagnosed only with FAB classification, and they werecorrected after chromosome analysis. Immunophenotype was performed on 10 cases, reveaing commonimmunoloic features, such as HLA-DR(-), CD14(-), CD33 (+) and CDl3 (+). Normal karyotppes werefound in 3 cases (3/35 ), translocation t(15; 17) was found in 31 cases (31/35) and variant transforation in 1cases (1/35). The clinic data of 21 cases were evaluated and 19(19/21 ) achieved a complete remission(CR).2 cases (one of them detnonsrated a more complex karyotype and the other a variant translocation) did notabtain CR. Conclusion: It is vere important to M3 that the combination of MIC techniques be applied to thediagnosis, classfication and evaluation of the prognostic.