烟草青枯病拮抗菌培养条件的研究

Studies on Culture Conditions for Suppressive Bacteria against Tobacco Wilt

为了开发有效防治烟草青枯病的生物有机肥，对与烟草青枯病拮抗效果较好的3株拮抗菌T3，T4，T5进行了培养条件研究，结果表明，3种菌最适培养条件为35℃，pH7．5，振荡培养28h时产菌量最多，4-28h为对数生长期。同时考察了拮抗菌利用堆肥生长的情况，3种菌均可利用堆肥生长，随着堆肥量的增多，生长量增加，如果同时适当增加培养基中的碳氮比，菌的生长更好。

To develop a biological manure which could effectively control the tobacco wilt, the cul- tural conditions of three antagonistic strains （T3,T4 and T5） against tobacco bacterial wilt were studied. The results indicated that the optimal initial pH value was 7.5 and the temperature was 35℃. The three strains reached the growth peak after 28 h,and their logarithm time of growth was in 4-28h. At the same time,the growth of the three strains utilizing compost was reviewed. All the three strains could utilize compost as substrate and grew better with increasing the content of the compost in the culture. If the C/N ratio in culture medium was increased properly,the microbial counts were increased.